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Optical Microscopy

We use lenses everyday whether we know it or not. There are two lenses in each
of our eyes: the cornea and the crystalline lens which focus light onto our
retinas. Lenses are used to not only correct our vision, but to enhance it by
letting us see worlds we normally wouldn't be able to see, via microscopes and
telescopes. The following are activities you can carry out at home.
Magnification and focal length
Follow the procedure in the
Lenses Activity and then return
here.
A simple microscope
You can use a drop of water on a clear plastic sheet to make a magnifying
glass. A water-drop is a plano-convex lens (one surface flat, one surface
curved out). Your image will not be very clear but you can magnify an image.
a) Put a drop of water on the clear sheet to form a water lens 5
millimeters (mm) or greater in diameter. Use a bright lamp (desk lamp) as a
light source and hold it over a paper so you can estimate the
focal length with a
ruler. You'll find the focal length to be about 5 millimeters -- maybe more,
maybe less. Can you estimate the magnification by measuring a known object
(hair is about 70 microns in diameter)? Compare the width of the image with
the diameter of your water lens which you can measure.
b) An improved water-drop magnifier. Take a stiff card 10
centimeters (cm) long and 3 cm wide - about the size of a microscope slide.
Punch out or cut a hole about 5 mm wide (standard paper hole punch is 7 mm in
diameter and maybe too big). Put a piece of clear plastic over the hole, tape
it down, and then put a drop of water on the plastic over the hole with the
droplet big enough to extend beyond the hole. Now you have a
water-Leeuwenhoeck microscope. See if you can determine focal length and
magnification.
Real and Virtual lmages
Here is a way to remember real and virtual images with a convex lens.
a) What to do
- Get the A lens from your
Optics Kit.
- Place the magnifying lens over the drawing of an arrow.
- Hold the magnifying lens at about arm's length away from
your eye, and slowly lift the magnifying lens off the picture.
- Keep lifting the magnifying lens until the image you are
watching turns upside down.
- Repeat for the B image
b) What happened
As you first began to move the magnifying lens further away
from the page, the image grew
larger. At one point the image became fuzzy and then came back into focus
upside down. This is because of the way the magnifying lens bends the light
traveling to your eye from the page. When the magnifying lens is close to the
page, you see what is known as a virtual image. This is an upright
image that is larger than the image on the page. As you move the magnifying
lens closer to your eye and away from the page, you see what is called a
real image, which is upside down. Look at the diagrams below to see
how the light is bent by the lens to form the two types of images you saw.
c) Summary: If the object is inside the focal plane (F) the image is
virtual and erect; if the object is outside the focal plane (F) the
image is real and inverted.
Continue on to the
Optical Microscopy Readings
Page
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Page authored by the ACEPT W3 Group Department of Physics and Astronomy, Arizona State University, Tempe, AZ 85287-1504 Copyright © 1995-2001 Arizona Board of Regents. All rights reserved.
Last modified 20 November 2001
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